Assay-kits

SensoLyte® 520 Cathepsin S Assay Kit Fluorimetric - 1 kit

443,00
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  • Cat.Number : AS-72099
  • Availability :
    In stock
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Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases.
The SensoLyte® 520 Cathepsin S Activity Assay Kit uses a 5-FAM/QXL™ 520 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the 5-FAM/QXL™ 520 based FRET peptide shows less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Specifications

Packaging
Kits components
  • Component A: 5-FAM/QXL™ 520, Cathepsin S substrate, Ex/Em=490 nm/520 nm upon cleavage: 1.6 mM, 50 µL Component B: 5-FAM, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Cathepsin S, human spleen: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin S Inhibitor: 1 mM, 10 µL Component F: DTT: 1 M, 100 µL
Chemistry
UniProt number
  • P25774
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 1 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

RAGE expression in tumor-associated macrophages promotes angiogenesis in glioma

Cancer Res . 2014 Oct 17 ; 74 7285 | DOI : 10.1158/0008-5472.CAN-14-1240

  • X. Chen
  • et al

Synthesis of vinyl sulfone-tethered proline derivatives as highly selective Cathepsin S inhibitors

Bull Korean Chem Soc . 2014 Feb 01 ; 35 345 | DOI : http://dx.doi.org/10.5012/bkcs.2014.35.2.345

  • M. Kim
  • et al

Synthesis of proline analogues as potent and selective cathepsin S inhibitors

Bioorg Med Chem Lett . 2013 Apr 16 ; 23 3140 | DOI : 10.1016/j.bmcl.2013.04.023

  • M. Kim
  • et al

Excessive activity of cathepsin K is associated with cartilage defects in a zebrafish model of mucolipidosis II

Dis Models Mech . 2012 Jan 01 ; 5 177 | DOI : 10.1242/dmm.008219

  • A.C. Petrey
  • et al

Excessive activity of cathepsin K is associated with cartilage defects in a zebrafish model of mucolipidosis II.

Dis Models Mech . 2011 Nov 01 ; 5(2) 177 | DOI : 10.1242/dmm.008219

  • A.C. Petrey
  • et al