SensoLyte® 520 Cathepsin S Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72099
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases.
The SensoLyte® 520 Cathepsin S Activity Assay Kit uses a 5-FAM/QXL™ 520 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the 5-FAM/QXL™ 520 based FRET peptide shows less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.


Kits components
  • Component A: 5-FAM/QXL™ 520, Cathepsin S substrate, Ex/Em=490 nm/520 nm upon cleavage: 1.6 mM, 50 µL Component B: 5-FAM, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Cathepsin S, human spleen: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin S Inhibitor: 1 mM, 10 µL Component F: DTT: 1 M, 100 µL
UniProt number
  • P25774
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Biomarker Target
Detection Method
Detection Limit
  • 1 ng/ml
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

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  • X. Chen
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