Assay-kits

SensoLyte® 520 Mouse Renin Assay Kit Fluorimetric - 1 kit

564.00
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  • Cat.Number : AS-72161
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    In stock
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Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I. Angiotensin I is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research.
The SensoLyte® 520 Mouse Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of mouse renin activity using a 5-FAM/QXL™520 FRET peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm.

Specifications

Packaging
Kits components
  • Component A: Mouse renin substrate 5-FAM/QXL™520 FRET peptide: 50 µL Component B: 5-FAM, fluorescence reference standard: 100 µM, 10 µL Component C: Mouse Prorenin: 0.5 mg/mL, 20 µL Component D: Assay buffer: 25 mL Component E: Trypsin Activation buffer: 300 µL Component F: Trypsin Inhibitor: 10 mM, 20 µL Component G: Renin Inhibitor Ac-HPFV- (Sta)-LF-NH2: 1 mM, 10 µL
Chemistry
UniProt number
  • P06281
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A, B, F, and G from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

VDR Attenuates Acute Lung Injury by Blocking Ang-2-Tie-2 Pathway and Renin-Angiotensin System

Mol Endocrinol . 2013 Nov 06 ; 2116 27(12) | DOI : 10.1210/me.2013-1146

  • J. Kong
  • et al

Primary aldosteronism and impaired natriuresis in mice underexpressing TGFβ1

Proc Natl Acad Sci U S A . 2013 Mar 15 ; 5600 110(14) | DOI : 10.1073/pnas.1302641110

  • M. Kakoki
  • et al

Angiotensin II receptor blocker improves the lowered exercise capacity and impaired mitochondrial function of the skeletal muscle in type 2 diabetic mice

J Appl Physiol  . 2013 Jan 17 ; 114(7) 844 | DOI : 10.1152/japplphysiol.00053.2012

  • S. Takada
  • et al

Single atom substitution in mouse protein kinase G eliminates oxidant sensing to cause hypertension

Nat Med . 2020 Mar 18 ; 18(2) 286 | DOI : 10.1038/nm.2603

  • O. Prysyazhna
  • et al

Influence of angiotensin II type 1 receptor-associated protein on prenatal development and adult hypertension after maternal dietary protein restriction during pregnancy

J Am Soc Hypertens . 2012 Aug 28 ; 6(5) 324 | DOI : 10.1016/j.jash.2012.07.001

  • K. Tsukuda
  • et al

Vitamin D receptor signaling in podocytes protects against diabetic nephropathy

J Am Soc Nephrol . 2012 Nov 02 ; 23(12) 1977 | DOI : 10.1681/ASN.2012040383

  • Y. Wang
  • et al

Viral transduction of renin rapidly establishes persistent hypertension in diverse murine strains.

Am J Physiol Regul Integr Comp Physiol . 2015 Jun 24 ; 309(5) R467 | DOI : 10.1152/ajpregu.00106.2015

  • S. Harlan
  • et al