SensoLyte® 520 Mouse Renin Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72161
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I. Angiotensin I is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research.
The SensoLyte® 520 Mouse Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of mouse renin activity using a 5-FAM/QXL™520 FRET peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm.


Kits components
  • Component A: Mouse renin substrate 5-FAM/QXL™520 FRET peptide: 50 µL Component B: 5-FAM, fluorescence reference standard: 100 µM, 10 µL Component C: Mouse Prorenin: 0.5 mg/mL, 20 µL Component D: Assay buffer: 25 mL Component E: Trypsin Activation buffer: 300 µL Component F: Trypsin Inhibitor: 10 mM, 20 µL Component G: Renin Inhibitor Ac-HPFV- (Sta)-LF-NH2: 1 mM, 10 µL
UniProt number
  • P06281
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

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