Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research. The SensoLyte® 520 Rat Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL™520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm.
Component A: Rat renin substrate 5-FAM/QXL™520 FRET peptide: 1 mM, 25 µL
Component B: 5-FAM, fluorescence reference standard: 1 mM, 10 µL
Component C: Recombinant rat renin: 40 µl
Component D: Assay buffer: 25 mL
Component E: Renin Inhibitor Ac-HPFV- (Sta)-LF-NH2: 1 mM, 10 µL
Storage & stability
Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.