Assay-kits

SensoLyte® 520 Rat Renin Assay Kit Fluorimetric - 1 kit

641.00
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  • Cat.Number : AS-72140
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    In stock
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Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research.
The SensoLyte® 520 Rat Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL™520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm.

Specifications

Packaging
Kits components
  • Component A: Rat renin substrate 5-FAM/QXL™520 FRET peptide: 1 mM, 25 µL Component B: 5-FAM, fluorescence reference standard: 1 mM, 10 µL Component C: Recombinant rat renin: 40 µl Component D: Assay buffer: 25 mL Component E: Renin Inhibitor Ac-HPFV- (Sta)-LF-NH2: 1 mM, 10 µL
Chemistry
UniProt number
  • P08424
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.4 ug/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

Effects of acute and chronic boysenberry intake on blood pressure and endothelial function in spontaneous hypertensive rats

J Nutr Sci Vitaminol  . 2014 Jan 01 ; 60(1) 43 | DOI : 10.3177/jnsv.60.43

  • A. Matsushima
  • et al

A novel vascular clip design for the reliable induction of 2-kidney, 1-clip hypertension in the rat

J Appl Physiol . 2011 Nov 10 ; 112(3) 362 | DOI : 10.1152/japplphysiol.01015.2011

  • S.P. Chelko
  • et al

Renin activates PI3K-Akt-eNOS signaling through the AT1 receptor and Mas receptor to modulate central blood pressure control in the nucleus tractus solitarii

Br J Pharmacol . ; 166(7) 2024 | DOI : 10.1111/j.1476-5381.2012.01832.x

  • W-H. Cheng
  • et al

Prevention of acute kidney injury in a rodent model of cirrhosis following selective gut decontamination is associated with reduced renal TLR4 expression

J Hepatol. . 2012 Jan 17 ; 56(5) 1047 | DOI : 10.1016/j.jhep.2011.11.024

  • N. Shah
  • et al