Assay-kits

SensoLyte® 520 TEV Protease Assay Kit Fluorimetric - 1 kit

364,00
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  • Cat.Number : AS-72227
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal.
The SensoLyte® TEV Protease Assay Kit uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL™ 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL™ 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly
proportional to the TEV protease quantity/activity.

Specifications

Packaging
Kits components
  • Component A: 5-FAM/QXL™ 520 TEV Substrate, Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component B: 5-FAM, Fluorescence Reference Standard, Ex/Em=490 nm/520 nm: 100 µM, 10 µL Component C: TEV Protease, Recombinant: 2 µG X 4 vials Component D: Assay buffer: 30 mL Component E: DTT, 1M: 30 µL
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store Components A, B, C, and E at -20°C; Protect Components A and B from light and moisture; Component D can be stored at room temperature for convenience
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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