SensoLyte® AFC Plasmin Activity Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72124
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis.
The SensoLyte® AFC Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity. The assay utilizes a fluorogenic peptide, which upon enzyme cleavage releases the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. The fluorescent signal can be measured at Ex/ Em =380 nm/500 nm. This assay can detect picogram level of plasmin activity.


Kits components
  • Component A: Plasmin substrate, Ex/Em=380 nm/500 nm upon cleavage: 3 mM, 50 µL Component B: AFC, fluorescence reference standard, Ex/Em=380 nm/500 nm: 3 mM, 10 µL Component C: Human plasmin: 250 µg/mL, 10 µL Component D: 2X Assay Buffer: 10 mL Component E: Plasmin Inhibitor: 1 mM, 10 µL Component F: Stop Solution: 5 mL
UniProt number
  • P00747
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D and F can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Biomarker Target
Detection Method
Detection Limit
  • 0.8 ng/ml
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

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