Assay-kits

SensoLyte® GST Activity Assay Kit Fluorimetric - 1 kit

273,00
Check your price
  • Cat.Number : AS-72157
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Quantity

GST (Glutathione S-Transferase) enzymes, a family of isozymes plays a crucial role in body defense mechanisms against toxins and carcinogens. GSTs carry out their functions mostly by mediating their conjugation with reduced glutathione on substrates such as pharmaceutical and environmental pollutants. The SensoLyte® GST Activity Assay Kit utilizes an optimized method for detection of enzyme activity in biological samples. The kit provides substrate for GST enzyme, which upon reaction with reduced glutathione yields a fluorescent glutathione adduct. This produces bright blue fluorescence that can be monitored at excitation/emission =390 nm/480 nm.

Specifications

Packaging
Kits components
  • Component A: GST Substrate: 1 vial Component B: GST Standard: 10 U/mL, 40 µL Component C: Assay buffer: 50 mL Component D: Reduced Glutathione: 20 mM, 300 µL Component E: DMSO: 100 µL
Properties
Absorbance (nm)
  • 390
Emission (nm)
  • 480
Storage & stability
Storage Conditions
  • Store Components A and D at -20°C. Store Component B at -80°C. Aliquot as needed to avoid multiple freeze-thaw cycles. Components C and E can be stored at room temperature for convenience. Protect Component A from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

Citations

Chronic anthracycline cardiotoxicity: molecular and functional analysis with focus on nuclear factor erythroid 2-related factor 2 and mitochondrial biogenesis pathways.

J Pharmacol Exp Ther . 2012 Aug 22 ; 343(2) 468 | DOI : 10.1124/jpet.112.198358

  • E. Jirkovsky
  • et al