SensoLyte® HABA Biotin Quantitation Kit Colorimetric, Large size - 1 kit

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  • Cat.Number : AS-72096-500
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay employs HABA (4’-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. The kit is best used to determine biotin concentration in the range from 2 to 16 µM. The assay can be performed in a cuvette or microplate format.


Kits components
  • Component A: Avidin: 4 bottles Component B: HABA assay buffer: 110 mL Component C: d-biotin: 100 µM, 1 mL
Absorbance (nm)
  • 500
Storage & stability
Storage Conditions
  • Store component A at -20°C. Store all other components at 4°C.
Biomarker Target
Detection Method
Detection Limit
  • 2-16uM
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

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