Assay-kits

SensoLyte® NADP/NADPH Assay Kit Colorimetric - 1 kit

363,00
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  • Cat.Number : AS-72205
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Nicotinamide nucleotides are key players in the energy transforming and oxidation-reduction reactions of a cell. NADP and NADPH act as allosteric effectors in modulating enzyme activities. The reducing power of NADPH is a required cofactor for enzymes that are pro-oxidants such as nitric oxide synthase and NADPH oxidase. NADP/NADPH ratio is involved in the control of the oxidative shunt. Each of these forms has a particular role during differentiation and similarity processes as well as being target functions in the cytotoxic action of ionizing radiations and alkylating agents. The SensoLyte® NADP/NADPH Assay Kit utilizes an optimized enzymatic recycling method for the quantification of NADP/NADPH without interfering with NAD/NADH. The final product of reaction, formazan, has blue color and can be detected at absorbance 565 nm. The intensity of the color produced is proportional to NADP/NADPH concentration.

Specifications

Packaging
Kits components
  • Component A: Reagent A: 180 µL Component B: Reagent B: 80 µL Component C: Assay Buffer: 50 mL Component D: Enzyme Cycling Mix: 80 µL Component E: NADP Standard: 1.5 mM, 20 µL Component F: NADP Extraction Buffer: 25 mL Component G: NADPH Extraction Buffer: 25 mL Component H: Stop solution: 20 mL
Properties
Absorbance (nm)
  • 565
Storage & stability
Storage Conditions
  • Store kit Components A, B and E at -20°C, protect from light. Store Component D at -80°C. Aliquot as needed to avoid multiple freeze-thaw cycles. Components C, F, G and H can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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