Assay-kits

SensoLyte® ONPG ß-Galactosidase Assay Kit Colorimetric - 1 kit

204,00
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  • Cat.Number : AS-72134
  • Availability :
    In stock
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β-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells. The SensoLyte® ONPG β-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl β-D-galactopyranoside (ONPG) to determine the activity of β-galactosidase in transfected cells or tissues. In the presence of β-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to β-galactosidase activity.

Specifications

Packaging
Kits components
  • Component A: Substrate solution: 50 mL Component B: ß -galactosidase enzyme: 0.1 mg/mL, 20 µL Component C: Lysis buffe:r 100 mL Component D: Stop solution: 30 mL Component E: DTT: 1 M, 5 mL
Chemistry
UniProt number
  • P16278
Properties
Absorbance (nm)
  • 420
Storage & stability
Storage Conditions
  • Store all components at -20°C. Keep component A away from light. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 1 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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