Assay-kits

SensoLyte® Rh110 DPPIV Assay Kit Fluorimetric - 1 kit

330,00
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  • Cat.Number : AS-72196-
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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DPPIV is a widely distributed serine protease that cleaves two amino acids from small peptides containing alanine or proline in the second position of the N-terminus of the peptide. DPPIV activity is especially critical for inactivation of GLP-1, a potent antihyperglycemic hormone, inducing glucose-dependent stimulation of insulin secretion. DPPIV inhibitors provide a potent treatment for type 2 diabetes prolonging the activity of GLP-1 and improving insulin secretion and blood glucose control regulation.
The SensoLyte® Rh110 DPPIV Assay Kit provides a convenient method for high throughput screening of DPPIV inhibitors and for continuous assay of DPPIV activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from reaction components.

Specifications

Packaging
Kits components
  • Component A: DPPIV Substrate, Ex/Em=490/520 nm upon cleavage: 55 µL Component B: Rh110, fluorescence reference standard, Ex/Em=490 nm/520 nm: 5 mM, 10 µL Component C: DPPIV, porcine kidney: 0.1 mg/mL, 15 µL Component D: Assay Buffer: 25 mL Component E: DPPIV Inhibitor: 10mM, 15 µL
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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