Assay-kits

SensoLyte® Rh110 Enterokinase Assay Kit Fluorimetric - 1 kit

422,00
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  • Cat.Number : AS-72210
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Enterokinase or enteropeptidase is a heterodimeric serine protease produced by cells in the duodenal wall. It converts trypsinogen to trypsin, indirectly activating a number of pancreatic digestive enzymes. Enterokinase is a key enzyme for intestinal digestion of proteins. Its deficiency may cause severe protein malabsorption leading to impaired development and growth. Enterokinase also plays an important role in acute experimental and clinical pancreatitis. The high degree of specificity exhibited by enteropeptidase for cleaving affinity or other tags from recombinant proteins makes it an ideal tool for use in biochemical applications.
The SensoLyte® Rh110 Enterokinase Assay Kit employs a fluorogenic substrate for the
detection of enterokinase activity. Upon protease cleavage, this substrate generates the Rh110
(rhodamine 110) fluorophore with bright green fluorescence that can be detected at
excitation/emission=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components. The assays are performed in a convenient 96-well microplate format.

Specifications

Packaging
Kits components
  • Component A: Rh110 Enterokinase substrate, Ex/Em=490 nm/520 nm upon cleavage: 2 mM, 50 µL Component B: Rh110, fluorescence reference standard, Ex/Em=490 nm/520 nm: 2 mM, 10 µL Component C: Recombinant bovine enterokinase: 40 µL Component D: 2X Assay Buffer: 25 mL Component E: Enterokinase Inhibitor: 50 mM, 20 µL
Chemistry
UniProt number
  • P98073
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components, except Component C, at -20°C. Store Component C at –80°C. Protect Components A and B from light and from moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.31 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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