Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis. The SensoLyte® Rh110 Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=490 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from reaction components.
Component A Plasmin substrate, Ex/Em=490 nm/520 nm upon cleavage 5 mM, 50 µL
Component B: Rh110, Fluorescence reference standard, Ex/Em=490 nm/520 nm: 5 mM, 10 µL
Component C: Human plasmin: 250 µg/mL, 10 µL
Component D: 2X Assay Buffer: 10 mL
Component E: Plasmin Inhibitor: 1 mM, 10 µL
Component F: Stop Solution: 5 mL
Storage & stability
Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Components D and F can be stored at room temperature for convenience.