In vivo oligonucleotides are the smart choice for antisense oligonucleotides or siRNA testing at a research level before entering into pre-clinical studies.
The production process of in vivo oligonucleotides includes the following steps: HPLC purification, desalting, sterile filtration and lyophilization. The specific production process has been endotoxin tested in order to ensure in vivo oligonucleotides are suitable for animal research. Endotoxins were undetectable using a chromogenic LAL endotoxin assay.
This purification process is only available for oligonucleotides shorter than 60 nucleotides.
Modified bases such as 2’OMe, LNA or MOE bases are available. Up to 5 Phosphorothioate linkages can be included in order to increase nuclease resistance and oligo stability.
Custom siRNA duplexes are delivered as :
- 2 complementary siRNA strands (19 to 25 RNA bases plus 2 x 3’-DNA overhang bases)
- Purified following the oligo process for in vivo use
- 100 % MALDI-TOF Mass Spectrometry controlled
In order to ensure maximum stability and, if not annealed, all siRNAs are provided lyophilized in individual tubes.
Free annealing on request (shipped with free annealing buffer and RNase-free water).
Please note that each single siRNA strand can be ordered separately.