Oligos

siRNA Duplex with in vivo purification process - 1 duplex

235,00
Check your price
  • Cat.Number : SR-IVGR-020
  • Availability :
    In production

Select specification

Delivered Quantity

In vivo oligonucleotides are the smart choice for antisense oligonucleotides or siRNA testing at a research level before entering into pre-clinical studies.

The production process of in vivo oligonucleotides includes the following steps: HPLC purification, desalting, sterile filtration and lyophilization. The specific production process has been endotoxin tested in order to ensure in vivo oligonucleotides are suitable for animal research. Endotoxins were undetectable using a chromogenic LAL endotoxin assay.

This purification process is only available for oligonucleotides shorter than 60 nucleotides.
Modified bases such as 2’OMe, LNA or MOE bases are available. Up to 5 Phosphorothioate linkages can be included in order to increase nuclease resistance and oligo stability.


Custom siRNA duplexes are delivered as :
- 2 complementary siRNA strands (19 to 25 RNA bases plus 2 x 3’-DNA overhang bases)
- Purified following the oligo process for in vivo use
- 100 % MALDI-TOF Mass Spectrometry controlled
In order to ensure maximum stability and, if not annealed, all siRNAs are provided lyophilized in individual tubes.
Free annealing on request (shipped with free annealing buffer and RNase-free water).
Please note that each single siRNA strand can be ordered separately.

Specifications

Storage & stability
Form
  • Dried
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (Please use Rnase free buffer - refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use
Codes
Code Nacres
  • NA.51

You may also be interested in the following product(s)

Example of a tube of Oligonucleotide with 2'O-MOE RNA Base

2'O-MOE RNA Base - 1 base

Cat.Number : BA-ME001-004
10,00
Tube of Control siRNA duplex negative control

Control siRNA duplex negative control - 5 nmol

Cat.Number : SR-CL000-005
64,00
Picture showing a Mu-mlV Reverse Transcriptase kit

Mu-MLV Reverse Transcriptase - 40000 U

Cat.Number : ME-0125-400
255,00