Labeling-detection

Streptavidin, HiLyte™ Fluor 750 conjugated - 1 mg

82,00
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  • Cat.Number : AS-60659-H750
  • Availability :
    In stock
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Quantity

HiLyte™ Fluor 750-streptavidin conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised streptavidin function. Spectrally similar to Cy®7 dye, HiLyte™ Fluor 750 is the longest-wavelength HiLyte™ Fluordye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte™ Fluor 647 or allophycocyanin (APC), facilitating multicolor analysis. With a peak excitation at ~754 nm, conjugates of HiLyte™ Fluor 750 dyes are well excited by a xenon-arc lamp or dye-pumped lasers operating in the 720–750 nm range. The fluorescence signal of HiLyte™ Fluor 750-conjugate can be observed at 778 nm with the excitation at 754 nm.

Specifications

Chemistry
Molecular Mass/ Weight
  • ~55.000
Properties
Absorbance (nm)
  • 754
Emission (nm)
  • 778
Quantity & Purity
Concentration
  • 1 mg/ml
Storage & stability
Form
  • In solution
Storage Buffer
  • 10 mM phosphate, 150 mM NaCl, pH 7.2, with BSA and 2 mM NaN3
Storage Conditions
  • The dye-protein conjugate is stable for 2~3 months at 4°C. For long-term storage, divide the solution into aliquots and store at -20°C or add an equal volume of glycerol (ACS grade or higher) and store the solution at -20°C without aliquoting. Avoid multiple thaw-freeze cycles. The product is stable for 1 year at -20°C.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • E. coli

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