PCR & qPCR

Takyon™ Dry One-Step RT Probe MasterMix Low Rox - 150 RXN (20 µL)

150,00
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  • Cat.Number : UFD-LPRT-C0101
  • Availability :
    In stock

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Quantity

Takyon™ Dry Low Rox One-Step RT Probe MasterMix dTTP contains the Takyon™ fusion enzyme for fast, consistent and sensitive results. It contains ROX passive reference for well to well signal normalization. The Takyon™ reagents are provided lyophilized in bottles of 50 (20 µL) RXN.

On the Mx3000P®, Mx3005® and Mx4000®, both MasterMixes with and without ROX passive reference can be used depending on your needs. If you do not perform ROX normalisation on your results, we advise you to use the Takyon Dry One-step RT Kits for Probe Assay - No ROX which have been validated on these thermocyclers. ; Other formulations are available on request

Specifications

Packaging
Kits components
  • 3 bottles of Lyophilized MasterMix (50 RXN) containing Takyon™ DNA polymerase, Euroscript II RT, RNase Inhibitor, dNTPs, MgCl2 (5.5 mM final concentration), Rox Passive reference, Stabilizers; 2 x 1,6 mL Buffer S (Blue cap); 1 x 2 mL PCR grade water (Clear cap)
Properties
Format
Color
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • Resuspend the Takyon™ Dry MasterMix dTTP as per follow: 1) Add the appropriate volume of buffer S or PCR grade water: - 2X : 525 µL PCR grade water or buffer S - 5X : 210 µL PCR grade water or buffer S 2) Close the bottle and stir gently for 15 sec
Storage Buffer
  • Dry, Buffer S or PCR grade water
Storage Conditions
  • Takyon™ Dry is stable for 18 months at 15-35°C dried. Once resuspended in PCR grade water, use upon resuspension. Once resuspended in Buffer S, it is stable for 24 hours at 4°C or 1 month at -20°C.
Activity
qPCR compatible thermocyclers
Application
Template type
  • RNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use

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