Takyon™ qPCR MasterMixes for Probe assays containing Low ROX passive reference are available in different formats from 1.5 ml to 10 x 7.5 ml. Takyon™ Kits deliver FAST, SENSITIVE and ACCURATE results on the following FAST & regular Cycling conditions
On the Mx3000P®, Mx3005® and Mx4000®, both MasterMixes with and without ROX passive reference can be used depending on your needs. If you do not perform ROX normalisation on your results, we advise you to use the Takyon Kits for Probe Assay - No ROX which have been validated on these thermocyclers. For carry-over prevention, combine any dTTP Mastermix with the new dUTP/UNG additive (RT-UTPUNG-020). To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon™ One-step kit converter for 150 rxns: UF-RTAD-D0101, for 750 rxns: UF-RTAD-D0701
Avoid repeated freeze-thaw cycles.
Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (red cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, ROX Passive reference, Stabilizers. 1x 1.5 ml 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (red cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, ROX Passive reference, Stabilizers. 1x 1.5 ml 50 mM MgCl2 (clear cap; optional use)
For long term storage the Takyon™ qPCR MasterMixes should be stored between -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable during 24 months. For short term storage the Takyon™ qPCR MasterMixes can be stored at 4 °C for 6 months.