Microtubule associated protein (Tau) is found predominantly in the central neural system and its major function is to promote assembly and to stabilize neuronal microtubules. Six isoforms of Tau were identified in humans that are differentiated by the exclusion or inclusion of exons 2, 3, and 10. Tau-441 is the longest of Tau isoforms that consists of 441 amino acids with a molecular mass of 45.8 kDa.
Tau441 fluorescent conjugates have been reported in various applications such as protein aggregation, cell uptake study and in vitro imaging.
The fluorescence of HiLyte™ Fluor 488-Tau conjugate can be observed at the excitation/emission wavelength of 490 nm/525 nm.
The recombinant human Tau (Tau-441) (GenBank Accession # AAC04279.1) was expressed and purified from E. coli and conjugated with the fluorescence dye HiLyte Fluor™ 488.