E.coli uracil-DNA glycosylase (UDG or UNG) hydrolyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases). The Uracil-N-Glycosylase (UNG) is an enzyme that hydrolyses all single-stranded and double-stranded DNA containing dUTPs. Consequently, if all PCR amplifications are performed in the presence of a dNTPs/dUTP blend, by carrying a UNG step before every run it is possible to get rid of any previous undesired PCR product containing dUTP.
Uracil-N-glycolsylase (UNG) can be combined with each dUTP containing qPCR Core kit or qPCR MasterMix UNG efficiently hydrolyzes the release of free uracil from uracil-containing single stranded or double stranded DNA, but not from oligomers (6 or less bases).
Each kit of 300 U contains: - 1 tube of 300 µL UNG (1u/µL) - 1 tube of reaction buffer 10x (200 mM Tris-HCl pH8.0, 10 mM EDTA, 10 mM DTT) Each kit of 1500 U contains: - 1 tube of 1,5 mL UNG (1u/µL) - 5 tubes of reaction buffer 10x (200 mM Tris-HCl pH8.0, 10 mM EDTA, 10 mM DTT)
For long term storage the UNG should be stored at -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 2 years. Avoid repetitive freeze-thaw cycles.
DNA containing dUTPs
0.01 u/µL final concentration
One unit is defined as the amount of enzyme that catalyses the release of 60 pmol of uracil per minute from doublestranded, uracil containing DNA.