E.coli uracil-DNA glycosylase (UDG or UNG) hydrolyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases). The Uracil-N-Glycosylase (UNG) is an enzyme that hydrolyses all single-stranded and double-stranded DNA containing dUTPs. Consequently, if all PCR amplifications are performed in the presence of a dNTPs/dUTP blend, by carrying a UNG step before every run it is possible to get rid of any previous undesired PCR product containing dUTP.
Uracil-N-glycolsylase (UNG) can be combined with each dUTP containing qPCR Core kit or qPCR MasterMix
UNG efficiently hydrolyzes the release of free uracil from uracil-containing single stranded or double stranded DNA, but not from oligomers (6 or less bases).