Are DNA or RNA standards preferred in qPCR?

RNA standards are preferred when starting from RNA because then the efficiency of the RT reaction can also be measured.

What is the longest RNA oligonucleotide you can synthesize?

RNA synthesis is not as straightforward as DNA synthesis....
However, we have synthesized RNA 70 mers for customers and these have been used successfully.

How do I avoid amplification of genomic DNA, which can be a contamination factor in my isolated RNA in RT qPCR?

In this way your amount of PCR products is directly related to the initial amount of cDNA, which is again directly related to the initial amount of RNA

How does Eurogentec quantify its oligos?

We carefully measure the OD (Optical Density) value for your custom oligonucleotide by measuring the absorption at 260 nm using an UV spectrometer....

How does Eurogentec select target sequences ?

We use bioinformatics tools to select siRNA oligos with characteristics perfectly matching the last experimental results in this field.

Does Eurogentec offer design for Real-Time probes?

Eurogentec is a specialist in the field of Real-Time PCR. Eurogentec offers a design service and can even do complete Real-Time projects. Learn more

What chemistry is used to synthesize Eurogentec's siRNA ?

Although similar to DNA synthesis, the additional 2'-OH group of RNA introduces considerable complexity to the RNA synthesis and requires a different protecting...
All RNA oligos at Eurogentec (including siRNA) are made using monomers containing a 2'-O-tertiary-Butyl-dimethylsylil (TBDMS) protecting group, assuring

How are Eurogentec's oligos made and which chemistry is used?

Our proprietary synthesis platforms provide computer-controlled oligo synthesis, cleavage, and deprotection in 4 different processing formats.

What is the typical extent of inhibition with Eurogentec's siRNA ?

We typically obtain 80 to 95 % inhibition at the mRNA level after 24 hours transfection. Inhibition at the protein level is highly dependent on the...