Assay Kits

Caspase Activity

Caspases play important roles in apoptosis and cell signaling

Apoptosis is a programmed, cell-autonomous death process. It plays important roles in a variety of physiological and pathological events1 , ranging from normal fetal development to diseases, such as cancer2 , organ failure and neurodegenerative diseases. During apoptosis, caspases execute the disassembly of the cellular components by proteolytic cleavage of a variety of substrates, such as poly-(ADP ribose) polymerase (PARP)3 , DNA-dependent protein kinase (DNA-PK), topoisomerases, and protein kinase C (PKC)δ. 4 At least ten caspases have been discovered. Some of caspases identify and cleave a specific peptide substrate, while others recognize the same peptide substrate4 .



1. Thornberry, N. A. et al. Science 281, 1312-1316 (1998).
2. Reed, J. C. J. Clin. Oncol. 17, 2941-2953 (1999).
3. Lazebnik, Y. A. et al. Nature 371, 346-347 (1994).
4. Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).

Assay Kits for caspase activity monitoring and inhibitors screening

Our Sensolyte® Caspase assay kits use fluorogenic substrates, where the sequence specific for one or several caspases is conjugated to AFC, AMC or Rh110. Upon protease cleavage, the fluorescence is released proportionally to the caspase activity.

Dye (donor) Donor Ex/Em
SensoLyte® AMC AMC

 351/430 nm

SensoLyte® AFC AFC

 382/480 nm

SensoLyte® Rh110 Rh110

 501/527 nm


The profiling kits contain two 96-well plates (8x12 strips) coated with eight well-known substrates for various caspases, while the Substrate sample kits contain the same peptides, but in bulk.

Two series of 8 peptides are available

8 peptides conjugated with AFC 8 peptides conjugated with AMC

References : AS-71116; AS-71117

SB1 Caspase-1 substrate Ac-YVAD-AFC
SB2 Caspase-1 substrate Ac-WEHD-AFC
SB3 Caspase-2 substrate Ac-VDVAD-AFC
SB4 Caspase-8 substrate Ac-IETD-AFC
SB5 Caspase-3/7 substrate Ac-DEVD-AFC
SB6 Caspase-3/7 substrate Z-DEVD-AFC
SB7 Caspase-9 substrate Ac-LEHD-AFC
SB8 Caspase-6 substrate Ac-VEID-AFC

References : AS-71120; AS-71121

SB1 Caspase-1 substrate Ac-YEVD-AMC
SB2 Caspase-1 substrate Ac-WEHD-AMC
SB3 Caspase-1/4 substrate Ac-YVAD-AMC
SB4 Caspase-3 substrate Ac-DMQD-AMC
SB5 Caspase-3/7 substrate Ac-DEVD-AMC
SB6 Caspase-6 substrate Ac-VEID-AMC
SB7 Caspase-8 substrate Ac-IETD-AMC
SB8 Caspase-9 substrate Ac-LEHD-AMC

  • Caspase Peptides

    Caspase 1, 3, 8 and 9 Substrates and inhibitors

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  • Custom Peptide Synthesis

    We produce standard and complex peptides

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