SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-71114
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    In stock
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Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
The SensoLyte® Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.


Kits components
  • Component A: Caspase-3/7 substrate, Ex/Em=380 nm/500 nm: 270 µL Component B: AFC, fluorescence reference standard, Ex/Em=380 nm/500 nm: 10 mM, 20 µL Component C: Caspase-3/7 inhibitor: 5 mM, 15 µL Component D: Assay Buffer: 30 mL Component E: DTT: 1 M, 1 mL Component F: 10X Lysis Buffer: 20 mL
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store components A, B, C, and E at -20°C. Components D and F can be stored at 4°C for convenience. Component A and B protect from light and moisture.
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

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