Peptides

Caspase 3 (Apopain) Substrate 1, chromogenic - 5 mg

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  • Cat.Number : AS-25263-5
  • Availability :
    In stock

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Quantity

Caspase-1 substrate with Km = 18 uM and kcat = 0.5 M-1s-1; Caspase-3 substrate with Km = 11 uM and kcat = 2.4 M-1s-1;Caspase-4 substrate with Km = 32 uM and kcat = 0.05 M-1s-1 Caspase-6 substrate with Km = 180 uM and kcat = 0.6 M-1s-1 ; Caspase-7 substrate with Km = 12 uM and kcat = 0.4 M-1s-1; Caspase-8 substrate with Km = 167 uM;
pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. pNA has maximum absorption around 408 nm.

Specifications

Chemistry
Sequence one letter code
  • Ac-DEVD-pNA
Sequence three letter code
  • Ac-Asp-Glu-Val-Asp-pNA
CAS registry number
  • 189950-66-1
Molecular Formula
  • C26H34N6O13
Molecular Mass/ Weight
  • 638.6
Properties
Absorbance (nm)
  • 405
Modification
Conjugation type
  • Conjugates
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • ≥ 95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.26

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Citations

Large-scale expression in Escherichia coli and efficient purification of precursor and active caspase-7 by introduction of thrombin cleavage sites

Protein Express Purification . 2009 Sep 25 ; 69(1) 29 | DOI : 10.1016/j.pep.2009.09.014

  • Y. Lee
  • et al

Synergism of Rana catesbeiana ribonuclease and IFN-gamma triggers distinct death machineries in different human cancer cells.

FEBS Lett . 2005 Jan 03 ; 579(1) 265 | DOI : 10.1016/j.febslet.2004.11.086

  • C-HA. Tang
  • et al

Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death.

FEBS Lett . 2002 Nov 20 ; 531(3) 421 | DOI : 10.1016/s0014-5793(02)03577-9

  • C-W. Wei
  • et al

Arsenic and fluoride induce neural progenitor cell apoptosis.

Toxicol Lett . 2011 Mar 23 ; 203(3) 237 | DOI : 10.1016/j.toxlet.2011.03.023.

  • R. Rocha
  • et al

Synergism of Rana catesbeiana ribonuclease and IFN-γ triggers distinct death machineries in different human cancer cells.

FEBS Lett . 2005 Jan 03 ; 579(1) 265 | DOI : 10.1016/j.febslet.2004.11.086

  • C-HA. Tang
  • et al