Assay-kits

SensoLyte® Homogeneous AMC Caspase-3/7 Assay Kit Fluorimetric - 1 kit

291.00
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  • Cat.Number : AS-71118
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
The SensoLyte®Homogeneous AMC Capase-3/7 Assay Kit uses Ac-DEVD-AMC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AMC generates the AMC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=354 nm/442 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.

Specifications

Packaging
Kits components
  • Component A: Caspase-3/7 substrate Peptide sequence=Ac-Asp-Glu-Val-Asp-AMC, Ex/Em=354 nm/442 nm upon caspase-3/7 cleavage: 270 µL Component B: AMC, fluorescence reference standard, Ex/Em=354 nm/442 nm: 10 mM DMSO solution, 20 µL Component C: Ac-DEVD-CHO, a known caspase-3/7 inhibitor: 5 mM DMSO solution 15 µL Component D: Assay Buffer: 30 mL Component E: DTT: 1 M, 1 mL Component F: 10X Lysis Buffer: 20 mL
Properties
Absorbance (nm)
  • 354
Emission (nm)
  • 442
Storage & stability
Storage Conditions
  • Store all kit components at -20°C. Protect Components A and B from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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