Peptides

Caspase 3 (Apopain) Substrate 1f, fluorogenic - 5 mg

172.00
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  • Cat.Number : AS-25273-5
  • Availability :
    In production

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Quantity

Caspase-3 substrate with kcat/Km = 1.3 ×106 M-1s-1, Caspase-7 substrate with kcat/Km = 1.3 ×104 M-1s-1;
AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.

Specifications

Chemistry
Sequence one letter code
  • Ac-DEVD-AFC
Sequence three letter code
  • Ac-Asp-Glu-Val-Asp-AFC
CAS registry number
  • 201608-14-2
Molecular Formula
  • C30H34F3N5O13
Molecular Mass/ Weight
  • 729.5
Properties
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Modification
Conjugation type
  • Fluorescent dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.26

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Citations

Large-scale expression in Escherichia coli and efficient purification of precursor and active caspase-7 by introduction of thrombin cleavage sites.

Protein Express Purification . 2009 Sep 25 ; 69(1) 29 | DOI : 10.1016/j.pep.2009.09.014

  • Y. Lee
  • et al