Standard “wobbles” are equimolar mixtures of two or more different bases at a given position within an oligonucleotide. Oligos with wobbles can be used as probes to hybridize to an unknown gene that encodes a known protein sequence, for random mutagenesis, and combinatorial chemistry.
Please always refer to the International Union of Biochemistry (IUB) base codes to introduce wobbles in your oligo sequences.
Mixed bases (also known as degenerate or wobble bases) follow the IUB codes:
Oligonucleotides made using mixed bases result in a final product that is a heterogeneous population of distinct species. MW, Tm and extinction coefficient may be strongly affected by mixed base addition. Rather than reporting the various values for each component, a single value is given.