ATTO-TEC dyes can be used as fluorescent labels for a large variety of biomolecules including oligonucleotides. ATTO dyes cover a wide spectral region.
ATTO dyes main features are :
- Strong absorption
- High fluorescence quantum yield
- High photostability
- Good water solubility
- Low triplet formationIdeally suited for bioanalytical applications
ATTO-TEC fluorescent labels are ideal for many bio-analytical applications. The absorption and fluorescence of some dyes are optimized for the red region of the spectrum, with the advantage of efficiently suppressing or by-passing any auto-fluorescence of biological samples. Other dyes exhibit a large shift of fluorescence relative to the absorption (Stokes-Shift). Again others are very hydrophilic, thus avoiding non-specific adsorption at bio-molecules and surfaces.
ATTO-TEC labels also find many applications in fluorescence spectroscopy. All dyes are fully characterized and can be used in time resolved spectroscopy, FRET (Fluorescence Resonance Energy Transfer) Real-Time qPCR assays applications as well as single- molecule detection.
ATTO 520 and ATTO 532 are fluorescent labels related to the well-known dye Rhodamine-6G.
ATTO 550 is a fluorescent label related to the well-known dyes Rhodamine-6G and Rhodamine B is the commercial alternative to NED.
ATTO 565, ATTO 590 and ATTO 594 are fluorescent labels belonging to the class of Rhodamine dyes. ATTO 594 is an alternative to Alexa Fluor® 594. ATTO 594 exhibits excellent water solubility, very good stability over a wide pH range, very good photo-stability, and high fluorescence quantum yield.
ATTO 610, ATTO 611X, ATTO 620, ATTO 633, ATTO 637, ATTO 647 and ATTO 647N are fluorescent labels for the red spectral region.
ATTO 633 is an alternative to Alexa Fluor 633, ATTO 633 shows strong absorption, very high fluorescence quantum yield, and very good stability in the pH-range 2 to 10.
ATTO 637 is another alternative to Alexa Fluor 633. ATTO 637 is more hydrophilic and better soluble in water.
ATTO 647N is an alternative to Cy® 5 and Alexa Fluor® 647. The characteristic features of ATTO 647N are strong absorption, extraordinary high fluorescence quantum yield and outstanding pH and photo-stability.
Suitable excitation sources for ATTO 637 are the 633 nm line of the He:Ne Laser or a Diode-Laser emitting at 635 nm. ATTO 647 and ATTO 647N are excited most efficiently in the range 615 - 660 nm. A suitable excitation source, for instance, is the 647 nm line of the Krypton-Ion laser or a Diode-Laser emitting at 650 nm.
ATTO 655, ATTO 680 and ATTO 700 fluorescence is excited most efficiently in the range 625 - 660 nm, 645 - 695 nm and 665- 715 nm respectively. A suitable excitation source for these dyes is the Krypton-Ion laser using the 676 nm line or a Diode-Laser emitting at 670 nm.
ATTO 725 together with ATTO 740 are fluorescent labels for the near infrared spectral region. Their fluorescence is excited most efficiently in the range 700 - 745 nm and 720 - 755 nm respectively.
ATTO 725 shows strong absorption and fluorescence. It can be excited with long-wavelength diode lasers.
ATTO 740 is another near-infrared emitting fluorophores.Its features are strong absorption and acceptable fluorescence.