Oligos

5' C2 Psoralen - 1 modification

145,00
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  • Cat.Number : MD-CN062-05004
  • Availability :
    In production

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Synthesis scale

Psoralen may be incorporated at the 5’ terminus only, using either a C2 or a C6 spacer arm. Psoralen intercalates between the two strands of a DNA duplex. When exposed to long-wavelength UV light, intercalated psoralen reacts with thymidine residues to form a covalent photoadduct. When attached to an oligonucleotide, psoralen thus forms interstrand crosslinks. The photoadducts can be reversed by short-wavelength UV light. The wavelength of the light used for the crosslinking experiment is critical to obtain a maximum diadduct.

Due to its relative hydrophobicity, psoralen oligos have the ability to cross membranes with relative ease. The C2 psoralen derivatization serves as an intercalating and cross-linking reagent in double-stranded oligonucleotides. The 6-atom spacer arm of psoralen C6 allows cross-linking with a triplex oligonucleotide strand.

Specifications

Chemistry
Spacers Sequence
  • 2 carbon atoms spacer
Molecular Formula
  • C16H15O7P
Molecular Mass/ Weight
  • 364,29
Modification
Modification Position
  • 5'
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use

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