Labeling-detection

5(6)-FAM [5-(and-6)-Carboxyfluorescein] UltraPure Grade Mixed Isomers

114,00
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  • Cat.Number : AS-81002-1
  • Availability :
    In stock

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Although FITC reagents have been more often used to prepare fluorescent bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents generate carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions, give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.

Specifications

Chemistry
CAS registry number
  • 76823-03-5
Molecular Formula
  • C21H12O7
Molecular Mass/ Weight
  • 376,32
Properties
Absorbance (nm)
  • 494
Emission (nm)
  • 519
Quantity & Purity
Purity
  • > 90% by TLC and HPLC
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C desiccated and protected from light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Measurement of flow velocity and inference of liquid viscosity in a microfluidic channel by fluorescence photobleaching.

Langmuir . 2014 Apr 14 ; 30(16) 4868 | DOI : 10.1021/la404891g

  • N.J. Carroll
  • et al