HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor 488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein. HiLyte™ Fluor 488 is more photostable than FITC, providing researchers with additional time for capturing image. Its fluorescence can be detected at the emission wavelength of 523±10 nm when excited at 499±10 nm. The HiLyte™ Fluor 488-protein conjugates are very stable and suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor 488-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 5 mg IgG.
Component A: HiLyte™ Fluor 488 SE Amino-reactive dye: 3 vials
Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL
Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns
Component D: DMSO solvent for preparing dye stock solution: 1 mL
Component E: 10x Elution buffer for eluting dye-protein conjugate: 30 mL
Storage & stability
Store all kit components at 4°C; Keep Component A away from light and protected from moisture