Labeling-detection

Protein A-HiLyte™ Fluor 488 Conjugate - 1 mg

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  • Cat.Number : AS-72235
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    In stock
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Protein A is a non-glycosylated cell wall protein of Staphylococcus aureus that can bind Fc part of immunoglobulin molecule of different species with strong affinity. Protein A consists of five IgG binding domains (A, B, C, D, and E), each is approximately 60 amino acids long with no cysteines present and two Staphylococcus aureus cell membrane binding domains (X and M).
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed in E.coli. Its apparent molecular mass is approximately 30,000 Da compared to the most reported 42,000 Da for the native Protein A that is found in Staphylococcus aureus. Recombinant Protein A conjugated with HiLyte™ Fluor-488 can be used for immunoglobulin detection in IHC and IF and for assay development. Green Fluorescence. Excitation/Emission wavelength= 499 nm/523 nm

Specifications

Chemistry
Molecular Mass/ Weight
  • ~30.000
Properties
Absorbance (nm)
  • 499
Emission (nm)
  • 523
Quantity & Purity
Concentration
  • 1 mg/ml
Purity
  • >95% by SDS-PAGE
Storage & stability
Form
  • In solution
Storage Buffer
  • 10mM phosphate, 150mM sodium chloride, pH 7.2, 0.1% sodium azide as preservative
Storage Conditions
  • Protein A-dye conjugate is stable for 2~3 months at 4°C. For long-term storage, divide the solution into aliquots and store at -20°C or add an equal volume of glycerol (ACS grade or higher) and store at -20°C without aliquoting. Avoid multiple thaw-freeze cycles. The product is stable for 1 year at -20°C.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • E. coli

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