Labeling-detection

AnaTag™ APC Labeling Kit - 1 kit

485,00
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  • Cat.Number : AS-72111
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Quantity

The AnaTag™ APC Labeling Kit is optimized for use in the conjugation of allophycocyanin (APC) to antibodies. APC is an ultra-sensitive, near-infrared fluorescent tracer with a high quantum yield (Ex/Em = 650 nm/660 nm). APC- labeled antibodies are used in applications such as flow cytometry and immunofluorescence. The AnaTagTM APC Labeling Kit contains SH-reactive APC. SMCC modified allophycocyanin reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol. AnaTagTM APC Labeling Kit contains a chemically cross-linked APC (CL-APC) that is much more stable than the native APC, but still retains the original spectroscopic properties. The amount of APC supplied in this kit is sufficient for labeling up to 1 mg of antibody. The kit provides all reagents, purification columns and a detailed step-by-step protocol. Cross-linked and SMCC-activated APC are also available as separate products. Kit Size: 1 labeling reaction (1x1 mg IgG)

Specifications

Packaging
Kits components
  • Component A: Activated CL-APC (SMCC activated cross-linked allophycocyanin): 2 mg, 10mg/mL Component B: DTT, reduces antibody: 0.1 mL Component C: Spin column which desalts protein by centrifugation: 1 column Component D: Wash tube to collect buffer from the spin column: 1 tube Component E: Collection tube to collect sample from the spin column: 1 tube Component F: Gravity column which desalts protein by gravity: 1 column Component G: DMSO: 1 mL Component H: NEM which blocks free sulfhydry
Properties
Absorbance (nm)
  • 650
Emission (nm)
  • 660
Storage & stability
Storage Conditions
  • Store all kit components at 4°C. Components B and H may be frozen for best performance. Keep the Component A away from light, do not freeze.
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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