Labeling-detection

AnaTag™ Europium Protein Labeling Kit - 1 kit

734,00
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  • Cat.Number : AS-72246
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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AnaSpec Europium (Eu) is an excellent tool for generating Eu-protein conjugates, and offers advantages over conventional fluorophores. Europium’s long-lived fluorescence and large Stoke’s shift allow for no background interference during assay measurements from the sample’s auto-fluorescence. The signal of Europium can be monitored at excitation/emission wavelengths = 325 nm/620 nm. Europium protein conjugates can be used in assay development for TR-FRET (time resolved-fluorescence resonance energy transfer) with red dyes as an acceptor such as HiLyte™ Fluor 647, either Cy5® or CyLyte, Alexa Fluor® 647 or APC. The kit has all the essential components for performing 1 conjugation reaction labeling up to 200 µg IgG and for purifying the Eu-protein conjugate. Kit Size: 1 labeling reaction (1 x 200 µg protein)

Specifications

Packaging
Kits components
  • Component A: Europium isothiocyanate, amine-reactive: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.6 mL Component C: Solvent buffer: 50 µL Component D: Spin column: 1 pre-packed column Component E: Elution buffer: 20 mL Component F: Wash tube: 1 tube Component G: Collect tube: 1 tube
Properties
Absorbance (nm)
  • 325
Emission (nm)
  • 620
Storage & stability
Storage Conditions
  • Store all kit components at 4°C. Keep component A away from light and protect from moisture. Component A may be frozen
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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