Labeling-detection

AnaTag™ Protein Labeling Kit for TR-FRET - 1 kit

826,00
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  • Cat.Number : AS-72247
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) involves an energy transfer between two fluorophores in close proximity: a donor molecule with long-lived fluorescence, such as terbium (Tb) and europium (Eu) and an acceptor molecule with short-lived fluorescence. TR-FRET pairs are chosen to achieve overlap of the donor emission and the acceptor absorbance spectra. Europium is usually paired with red-shifted dyes (allophycocyanine, either Cy5®, or CyLyte, or Alexa Fluor® 647) as energy acceptors. AnaTag™ Protein Labeling Kit for TR-FRET provides Europium (Eu) dye for generating Eu-protein conjugates, and acceptor red dye HiLyte™ Fluor 647. When Eu conjugates are used in TR-FRET with H647-labeled reagents the signal can be detected at excitation/emission wavelengths = 325 nm/667 nm.
Kit Size: 1 conjugation reaction with Eu and 1 conjugation reaction with HiLyte Fluor™ 647. Each conjugation reaction can label up to 200 ug proteins (IgG).

Specifications

Packaging
Kits components
  • Component A: Europium isothiocyanate, amine-reactive: 1 vial Component B: HiLyte™ Fluor 647, SE, amine-reactive: 1 vial Component C: Reaction buffer for pH adjustment of the conjugation reaction: 0.8 mL Component D: Solvent buffer: 50 µL Component E: DMSO: 200 µL Component F: Spin column: 2 pre-packed columns Component G: Elution buffer: 20 mL Component H: Wash tube: 2 tubes Component I: Collect tube: 2 tubes
Properties
Absorbance (nm)
  • 325
Emission (nm)
  • 667
Storage & stability
Storage Conditions
  • Store all kit components at 4°C. Keep component A and B away from light and protect from moisture. Component A and B may be frozen.
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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