Labeling-detection

Streptavidin, Europium labeled

372,00
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  • Cat.Number : AS-72252-50
  • Availability :
    In stock
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TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) combines advantages of time resolved fluorescence and FRET. Energy is transferred from a long-lived lanthanide chelate, such as Europium to an acceptor molecule with short-lived fluorescence. Eu-labeled donors have a longer lasting fluorescence signal than standard fluorescence dyes, which allows for measurements to be taken after decay of sample background autofluorescence, leaving only the TR-FRET signal, essentially free of background interference. Streptavidin Europium conjugate has been optimized in Eu/protein labeling ratio to ensure high signal and uncompromised streptavidin function. This product is stable in buffers with pH from 5.5 to 9.0, containing up to 20mM EDTA or DTT, and up to 1% Triton X-100 without significant loss of the luminescent activity of the conjugate.

Specifications

Chemistry
Molecular Mass/ Weight
  • ~55.000
Properties
Absorbance (nm)
  • 325
Emission (nm)
  • 620
Quantity & Purity
Concentration
  • 0.5 mg/ml
Storage & stability
Form
  • In solution
Storage Buffer
  • 10 mM phosphate, 150 mM NaCl, 0.05% Proclin-300, pH 7.2
Storage Conditions
  • Europium-streptavidin conjugate is stable for 1~2 months at 4°C. For long-term storage, divide conjugate into aliquots and store at -20 °C or add an equal volume of glycerol (ACS grade or higher) and store the solution at -20°C without aliquoting. Avoid multiple thaw-freeze cycles. The product is stable for 1 year at -20°C. Protect Eu-streptavidin conjugate from heat and light. Avoid buffers that contain Fe and Mn ions.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • E. coli

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