Labeling-detection

AnaTag™ R - PE Labeling Kit - 1 kit

485,00
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  • Cat.Number : AS-72113
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    In stock
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The AnaTag™ R-PE Labeling Kit is optimized for use in the conjugation of R-Phycoerythrin (R-PE) to antibodies. R-PE, a fluorescent protein from phycobiliprotein family, has broad absorption bands with peaks at 565 nm (eM =1.96 X 106 M-1cm-1), 498 (eM =1.53 X 106 M-1cm-1), and 539 nm (eM =1.62 X 106 M-1cm-1); it therefore can be excited with versatile excitation sources. Its maximal emission can be detected at 578 nm. The broad excitation spectrum also provides the advantage of multi-color immunofluorescent staining or cell sorting. R-PE and the closely related B-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. The AnaTagTM R-PE Labeling Kit contains SH-reactive R-PE. SMCC modified R-PE reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol. The amount of R-PE supplied in this kit is sufficient for labeling up to 1 mg of antibody. The kit provides all reagents, purification columns and a detailed step-by-step protocol. Native and SMCC-activated R-PE are also available as separate products.

Specifications

Packaging
Kits components
  • Component A: Activated R-PE (SMCC activated cross-linked R-Phycoerythrin): 3 mg, 10 mg/mL Component B: DTT: 0.1 mL Component C: Spin column: 1 column Component D: Wash tube: 1 tube Component E: Collection tube: 1 tube Component F: Gravity column: 1 column Component G: DMSO: 1 mL Component H: NEM to block free sulfhydryl groups: 1 vial Component I: Reaction buffer for conjugation of B-PE with protein: 100 mL
Properties
Absorbance (nm)
  • 565
Emission (nm)
  • 578
Storage & stability
Storage Conditions
  • Store all kit components at 4°C. Components B and H may be frozen for best performance. Keep the Component A away from light, do not freeze.
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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