Labeling-detection

Collagen (Type IV), FAM conjugated - 1 mg

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  • Cat.Number : AS-85112
  • Availability :
    In stock
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In this protease substrate, collagen is heavily labeled with a fluorescein derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. Collagen, Type IV should be particularly useful in the development of HTS assays for screening Gelatinase A (MMP-2) and Gelatinase A inhibitors, as well as for other gelatinases and collagenases that specifically degrade (Type IV) Collagen. Additionally, lightly fluorescein-labeled collagen may be useful for continuous assays monitored by fluorescence polarization.

Specifications

Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Quantity & Purity
Concentration
  • 1 mg/ml
Storage & stability
Form
  • In solution
Resuspension condition
  • Assay buffer, pH>7
Storage Buffer
  • 0.01 M acetic acid
Storage Conditions
  • To be stored at acidic pH at -20°C
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • human
Codes
Code Nacres
  • NA.32

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