Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion is well known. MMP-2 (gelatinase A) is proposed as a potential therapeutic target. The SensoLyte® 490 MMP-2 Assay Kit is a complete assay system designed to continuously analyze MMP activities or to screen MMP-2 inhibitors by using a fluorogenic MMP-2 substrate. Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-2 activity, please choose the SensoLyte® Plus MMP-2 Assay Kit, Cat# AS-72224.
Component A: MMP-2 substrate EDANS/DABCYL FRET peptide Ex/Em=340 nm/490 nm upon cleavage: 270 µL
Component B: EDANS, fluorescence reference standard Ex/Em=340 nm/490 nm: 1 mM DMSO solution, 10 µL
Component C: APMA, 4-aminophenylmercuric acetate (Caution: Contains organic mercury and must be handled with care. Dispose according to local regulations): 1 M, 100 µL
Component D: Assay buffer: 60 mL
Component E: Stop solution: 30 mL
Storage & stability
Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.