PCR & qPCR

dUTP/UNG Additive for dTTP Kit Conversion

81,00
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  • Cat.Number : RT-UTPUNG-020
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Size

Quantity

E. coli Uracil-N-glycosylase (UNG) hydrolyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases).

By using the dUTP/UNG blend, easily convert any dTTP MasterMix into a carryover prevention compatible kit in a single pipetting step.

Procedure
Convert a dTTP MasterMix kit before first use:
- Thaw the MasterMix and the vial containing dUTP/UNG at RT
- Add the dUTP/UNG blend to the MasterMix vial as follow:

- 40 µL into 1.5 mL of 2X MasterMix
- 40 µL into 0.6 mL of 5X MasterMix
- 67 µL per 1 mL tube of 5X MasterMix
- 200 µL per 7.5 mL bottle of 2X MasterMix

- Gently homogenize the MasterMix by swirling the vial
- Follow MasterMix storage recommendation for the converted kit


Convert a qPCR reaction into a carryover ready assay (for Core kit):
- Thaw all required reagents
- Add 0.25 µL of dUTP/UNG per 20 µL reaction (final volume - refer to the TDS of the corresponding Core kit)
- Gently homogenize the Mix by pipetting or inverting the tube a few times. Spin down.
- Start the reaction

Core kits always contain dUTP/UNG blend. No need to buy extra kit converter.

Specifications

Packaging
Kits components
  • 150 rxns kit contains: One tube containing 40 µL of dUTP/UNG Blend 750 rxns kit contains each: one tube (200 µL) of dUTP/UNG Blend 1250 rxns kit contains each: One tube containing 335 µL of dUTP/UNG Blend
Properties
Color
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the dUTP/UNG blend should be stored at -20°C in a constant temperature freezer. When stored under these conditions the reagent is stable for 2 years. Avoid repetitive freeze-thaw cycles.
Activity
Usage
  • Research use

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