PCR & qPCR

Takyon™ ROX SYBR 2X MasterMix dTTP blue

364.00
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  • Cat.Number : UF-RSMT-B0701
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Select specification

Size

Quantity

Takyon™ qPCR Kits for SYBR® assays containing ROX passive reference are available in different MasterMixes formats from 1.5 ml to 10 x 7.5 ml. Takyon™ qPCR Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & regular conditions

For carry-over prevention, combine any dTTP MasterMix with the new dUTP/UNG additive for 150 rxns: RT-UTPUNG-005, for 750 rxns: RT-UTPUNG-020
To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon™ One-step kit converter for 150 rxns: UF-RTAD-D0101, for 750 rxns: UF-RTAD-D0701

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (blue cap - amber tube) containing Takyon™ DNA polymerase, MgCl2 (2.5mM final concentration), SYBR Green®, dNTPs, ROX Passive reference, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (blue cap - amber vial) containing Takyon™ DNA polymerase, MgCl2 (2.5mM final concentration), SYBR Green®, dNTPs, ROX Passive reference, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use)
Properties
Format
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Takyon™ Rox SYBR® MasterMix dTTP blue should be stored at a temperature between -15°C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 12 months. For short term storage the Takyon™ Rox SYBR® MasterMix dTTP blue can be stored at 4 °C for 6 months. Product must be stored in the dark.
Activity
qPCR compatible thermocyclers
Application
Template type
  • cDNA/DNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

Variations in facial conformation are associated with differences in nasal microbiota in healthy dogs

BMC Veterinary Research . 2021 Nov 24 ; (2021) 17:361 1-16 | DOI : https://doi.org/10.1186/s12917-021-03055-w

  • Emilie Vangrinsven
  • et al

Host Defense Effectors Expressed by Hemocytes Shape the Bacterial Microbiota From the Scallop Hemolymph

frontiers in Immunology . 2021 Nov 12 ; (2020) 11:599625 | DOI : https://doi.org/10.3389/fimmu.2020.599625

  • Roxana Gonzàlez

Nitric Oxide Impacts Human Gut Microbiota Diversity and Functionalities

American Society for Microbiology . 2021 Sep 14 ; 5 | DOI : https://doi.org/10.1128/mSystems.00558-21

  • Marion Leclerc
  • et al

Establishment of a second generation homozygous CRISPRa human induced pluripotent stem cell (hiPSC) line for enhanced levels of endogenous gene activation

ScienceDirect . 2021 Aug 26 ; 56 (2021) 1025182 | DOI : https://doi.org/10.1016/j.scr.2021.102518

  • Eric Schoger
  • et al

Gut microbiota-derived short-chain fatty acids regulate IL-17 production by mouse and human intestinal γδ T cells

Cells Reports . 2021 Jul 06 ; 109332 | DOI : https://doi.org/10.1016/j.celrep.2021.109332

  • Louise Dupraz
  • et al

Clinical and in Vitro Evidence against Placenta Infection at Term by Severe Acute Respiratory Syndrome Coronavirus 2

ScienceDirect . 2021 Jun 08 ; 9 1610-1623 | DOI : https://doi.org/10.1016/j.ajpath.2021.05.009

  • Arthur Colson
  • et al

Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs

Theoretical and Applied Genetics . 2021 May 02 ; (2021) 134 2495-2515 | DOI : https://doi.org/10.1007/s00122-021-03838-7

  • Peihong Fang
  • et al