Labeling-detection

HiLyte™ Fluor 532 succinimidyl ester (SE) - 1 mg

230,00
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  • Cat.Number : AS-89341
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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HiLyte™ Fluor 532 dyes are new members of our proprietary HiLyte™ Fluor series with fluorescence excitation and emission maxima of ~545 nm and ~565 nm, respectively. The dyes are based on a new class of cyanine dyes developed by AnaSpec scientists. Unlike traditional cyanine dyes, HiLyte™ Fluor 532 dyes are not only highly water-soluble, pH-insensitive but also photostable. HiLyte™ Fluor 532 is suited for the frequency-doubled Nd:YAG laser line (Flow cytometry laser line: 532 nm; Microscopy laser line: 532 nm) and is a direct replacement for: Alexa Fluor® 532, Atto 532. HiLyte™ Fluor 532 has Extinction Coefficient (M-1cm-1) = 171,000 and Fluorescence quantum yield = 0.26. HiLyte™ Fluor 532 acid, SE is an amine reactive reagent for labeling peptides, proteins and antibodies.

Specifications

Chemistry
Molecular Mass/ Weight
  • 875.4
Properties
Absorbance (nm)
  • 545
Emission (nm)
  • 565
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO: Prepare 10 mg/ml dye stock solution in high quality anhydrous DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C desiccated and protected from light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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