HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy®3 dyes, resulting in an optimal match to filters designed for Cy®3 dyes. HiLyte™ Fluor 555 is more photostable than Cy®3, providing researchers with additional time for image capture. Additionally, our in-house data indicated that HiLyte™ Fluor 555 is superior to Cy®3 in fluorescence polarization-based assays. Extinction Coefficient (M-1cm-1): 150,000; Fluorescence quantum yield: 0.10; Fluorescence Life Time (ns): 0.3
DMF or DMSO: Prepare 10 mg/ml dye stock solution in high quality anhydrous DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.