Oligos

Ion-Exchange HPLC (IEX-HPLC) - 1 purification

20,00
Check your price
  • Cat.Number : PU-HPLIE-004
  • Availability :
    In production

Select specification

Synthesis scale

The main advantage of HPLC (high-pressure liquid chromatography; High-performance liquid chromatography) purification is the monitoring of the purity level. It is recommended for applications such as diagnostic primers and antisense probes.

Ion-Exchange chromatography (IEX-HPLC) method is based on a resin (generally sugar polymer) carrying positively (anion-exchanger) or negatively (cation exchanger) charging groups. Molecules to be isolated are bound on the support by electrostatic interactions. Fixed compounds are then eluted by addition in the mobile phase of competitive ions. On the basis that oligonucleotides differing in their sequence possess a different charge, anion-exchange resins can be used to separate the full-length oligonucleotides from failure materials. The resolution of this technique is very high but, as the RP-HPLC, its use is limited to purification of rather short oligonucleotides (up to 60 bases).

Specifications

Quantity & Purity
Purity
  • <85%

You may also be interested in the following product(s)

Example of a tube of Oligonucleotide constitued by DNA Base

DNA Base - 1 base

Cat.Number : BA-DN001-001
0,24
Picture representing oligonucleotides MALDI-TOF MS analysis

MALDI-TOF MS - 1 QC

Cat.Number : AD-QCMMS-001
10,00
Picture representing oligonucleotides aliquoting

Oligo aliquots in X tubes - 1 aliquoting

Cat.Number : AD-PKALQ-001
10,00