MapMarker® are sets of high quality fluorescently labelled DNA fragments designed to provide consistent intensities and migration patterns for DNA sizing standards and are compatible with all fluorescent-based separation instruments systems including capillary electrophoresis. MapMarker® DNA fragments are uniformly spaced to provide accurate base calling and precision sizing of samples.
MapMarker® contain singular DNA strands with a single fluorophore and bands ranging from 35 to 1500 bases depending on the MapMarker®. Single strands are labeled with a single dye. The products are available with all of the most commonly used fluorescent dyes, including rhodamine, tetramethylrhodamine, x-rhodamine and fluorescein. Custom configurations and dyes are also available.
MapMarker® are evaluated for resolution, intensity and background on denaturing polyacrylamide sequencing gels using laser excitation. MapMarker® are free of extraneous peaks over their entire sizing range and are free of "pull up" signal in other channels. MapMarker® produce consistent within run and between run sizing within their size range and reproducible runs in gels or capillary-based systems. MapMarker® may be used under either denaturing or nondenaturing conditions.
The markers are fully compatible with all current methodology, equipment and software. It can be used as well on gels as on capillary-based systems. All MapMarkers are at 8 fmoles/band/µl.
Specially designed for microsatellite sizing standards
Made up of single DNA strands of exact size
Regular spaced bands from 50 up to 1000 bases
Labelled with FAM, TAMRA, ROX
May be used under either denaturing or non denaturing conditions
Stable for a minimum of 18 months if stored at 4 °C in dark