Protein

Mouse Prorenin, recombinant - 20 µg

259.00
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  • Cat.Number : AS-72174
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Quantity

The renin–angiotensin system (RAS) plays a central role in the regulation of blood pressure and electrolyte homoeostasis. An overactive renin-angiotensin system leads to hypertension. As a result, renin is an attractive target for the treatment of this disease

Recombinant mouse prorenin is produced in HEK cells and purified by chelated metal affinity chromatography. It contains an 8x-Histidine tag at the C terminus. The apparent Mr of recombinant enzyme on SDS-PAGE is 41.7 kDa. Prorenin, the precursor of renin, is a glycosylated aspartic protease that consists of 2 homologous lobes. Prorenin can be activated with trypsin. Its activity can be measured in a FRET-based enzymatic assay

Specifications

Chemistry
UniProt number
  • P06281
Molecular Mass/ Weight
  • 41.7 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 0.5 mg/ml
Purity
  • ≥95% by SDS-PAGE
Storage & stability
Form
  • In solution
Storage Buffer
  • 50 mM Tris-HCl, pH 8.0
Storage Conditions
  • Store at -80°C. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of mouse renin FRET substrate 5-FAM/QXLTM 520 per minute at pH 7.5 at 25° C.
Specificity
  • Mouse prorenin
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • mouse
Codes
Code Nacres
  • NA.27

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