PolyAcrylamide Gel Electrophoresis (PAGE) - 1 purification

Check your price
  • Cat.Number : PU-PAGE0-004
  • Availability :
    In production

Synthesis scale

Alternative choices

In PAGE Purification method, oligonucleotides are loaded on a polyacrylamide gel with the best appropriated percentage of acrylamide to purify the desired full-length oligonucleotide. The major product obtained is the slowest migrating band, which is excised out of the gel under low-intensity UV shadowing. The oligonucleotide is eluted from the gel slice then precipitated with ethanol, quantified and packaged.

In most cases, full-length (n) oligonucleotide can be separated from oligonucleotides only one base shorter (n-1). Due to its excellent resolution, denaturing acrylamide gel electrophoresis provides a higher degree of purity than HPLC (purity level up to 90 % can be achieved) but yield obtained is lower than other purification techniques. Nevertheless PAGE is the best suitable method to purify oligonucleotides longer than 80 bases or intended for Gel-shift assays.


Quantity & Purity
  • <90%
Code Nacres
  • NA.51

You may also be interested in the following product(s)

Example of a tube of Oligonucleotide constitued by DNA Base

DNA Base - 1 base

Cat.Number : BA-DN001-001
0.34 Excl. Tax
Picture representing oligonucleotides MALDI-TOF MS analysis


Cat.Number : AD-QCMMS-001
14.10 Excl. Tax
Picture representing oligonucleotides aliquoting

Oligo aliquots in X tubes (maximum 10) – 1 aliquoting

Cat.Number : AD-PKALQ-001
13.00 Excl. Tax