Assay-kits

SensoLyte® 390 Cathepsin D Assay Kit Fluorimetric - 1 kit

381,00
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  • Cat.Number : AS-72098
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. It is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.
The SensoLyte® 390 Cathepsin D Activity Assay Kit uses a Mca/Dnp FRET peptide for measurement of enzyme activity. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm.

Specifications

Packaging
Kits components
  • Component A: Mca/Dnp, Cathepsin D substrate, Ex/Em=330/390 nm upon cleavage: 1.6 mM, 50 µL Component B: Mca fluorescence reference standard, Ex/Em=330/390 nm: 2 mM, 10 µL Component C: Cathepsin D, bovine spleen: 0.1 mg/mL, 20 µL Component D: Assay Buffer: 20 mL Component E: Pepstatin A: 4 mM, 100 µL Component F: DTT: 1 M, 100 µL
Chemistry
UniProt number
  • P07339
Properties
Absorbance (nm)
  • 330
Emission (nm)
  • 390
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Multiple A2E treatments lead to melanization of rod outer segment–challenged ARPE-19 cells

Mol Vis . 2014 Mar 14 ; 20 285 | DOI : PMC3955416

  • E. Poliakov
  • et al