Assay-kits

SensoLyte® 520 BACE2 Activity Assay Kit Fluorimetric - 1 kit

422,00
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  • Cat.Number : AS-72225
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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BACE2 (β-Secretase-2, Memapsin-1) belongs to the family of transmembrane aspartic proteases. BACE2 has approximately 75% homology with BACE1 (β-Secretase-1) and is similar in that both cleaves Amyloid Precursor Protein (APP) at β-site generating N-terminal part of beta-Amyloid peptide. Unlike BACE1 that is highly expressed in pancreas and brain tissue, BACE2 is more widespread and can be found in many peripheral tissues at higher levels compared to BACE1. BACE2 is one of the highest efficiency beta-amyloid peptide degrading enzyme that is less active only to insulin degrading enzyme (IDE); therefore, targeting BACE2 is considered a potential therapeutic strategy for the prevention and treatment of Alzheimer's disease.
The SensoLyte® 520 BACE2 Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of BACE2 inhibitors. It contains a QXL® 520/ HiLyte™ Fluor488 FRET substrate, derived from a peptide sequence surrounding cleavage site of BACE2. QXL® 520 quenches HiLyte™ Fluor488 fluorescence in the intact FRET peptide. Active BACE2 cleaves FRET substrate into two separate fragments resulting in the release of HiLyte™ Fluor488 fluorescence that can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of HiLyte™ Fluor488 is less interfered by the autofluorescence of components in biological samples and test compounds. Kit Size: 100 assays (96-well plate)

Specifications

Packaging
Kits components
  • Component A: HiLyte™ Fluor488/QXL™-520 BACE2 substrate, Ex/Em=490/520 nm upon cleavage: 1 mM, 50 µL Component B: HiLyte™ Fluor 488, fluorescence reference standard, Ex/Em=490/520 nm: 1 mM, 15 µL Component C: Assay Buffer: 30 mL Component D: BACE2 Inhibitor: 50 µM, 15 µL Component :E Stop Solution: 10 ml
Chemistry
UniProt number
  • Q9Y5Z0
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20 C; Protect Components A, B, and D from light and moisture; Component C can be stored at 4 C for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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