Assay-kits

SensoLyte® 440 West Nile Virus Protease Assay Kit Fluorimetric - 1 kit

462,00
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  • Cat.Number : AS-72079
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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West Nile virus (WNV) causes severe neurological disease and fatalities in both human and animal hosts. There is currently no effective vaccine or antiviral drug to protect against WNV infection. The West Nile virus NS3 protease, essential for the proteolytic processing of the viral polyprotein precursor, is a promising target for the development of anti-WNV drugs.
The SensoLyte® 440 West Nile Virus Protease Assay Kit provides a convenient homogenous assay for high throughput screening of WNV protease inhibitors and for continuous assay of NS3 protease activity using a fluorogenic Pyr-RTKR-AMC peptide. Upon NS3 protease cleavage, Pyr-RTKR-AMC generates the AMC fluorophore that has bright blue fluorescence and can be detected at excitation/emission=354 nm/442 nm.

Specifications

Packaging
Kits components
  • Component A: Pyr-RTKR-AMC, WNV protease substrate, Ex/Em=354 nm/442 nm: 5mM DMSO solution, 250 µL Component B: AMC, fluorescence reference standard, Ex/Em=354 nm/442 nm: 5 mM DMSO solution, 10 µL Component C: Assay Buffer: 2 x 50 mL Component D: WNV Protease Inhibitor undeca-D-Arg-NH2: 1 mM DMSO solution, 10 µL
Properties
Absorbance (nm)
  • 354
Emission (nm)
  • 442
Storage & stability
Storage Conditions
  • Store all kit components at -20°C. Protect Components A and B from light and from moisture. Component C can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Discovery, Synthesis, and in vitro Evaluation of West Nile Virus Protease Inhibitors Based on the 9,10‐Dihydro‐3H,4aH‐1,3,9,10a‐tetraazaphenanthren‐4‐one Scaffold

Chem Med Chem . 2010 Apr 30 ; 7(7) 1210 | DOI : https://doi.org/10.1002/cmdc.201200136

  • S. Samanta
  • et al

Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication

J Virol. . 2009 Sep 30 ; 83(24) 12895 | DOI : 10.1128/JVI.00942-09

  • R. Assenberg,
  • et al