Protein

West Nile Virus NS3 Protease, recombinant

75,00
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  • Cat.Number : AS-72081-5
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

Size

Quantity

West Nile virus (WNV) is a member of the flavivirus genus, which contains many significant human pathogens including Dengue virus (Den), Japanese Encephalitits virus (JE), and Yellow Fever virus (YF). WNV is a small, enveloped virus with a single stranded, positive sense 11kb RNA genome, which encodes a polyprotein precursor. This polyprotein must be cleaved co- and post-translationally to produce ten functional proteins: three structural (C, prM and E) and 7 nonstructural (NS1, NS2A, NS2B, NS3, NS4A,NS4B and NS5). WNV NS3 protease is absolutely essential (along with viral-encoded cofactor NS2B) for post-translational cleavage and viral replication. As a result, this protease is a potential therapeutic target

His-tagged recombinant WNV NS3 protease (residues 1 to 184) was expressed as a fusion protein with the cofactor NS2b (residues 49 to 96) and a linker sequence (GGGGSGGGG) in E. coli. The apparent Mr on SDS-PAGE is 32-kDa. Its activity can be measured by FRET peptides

Specifications

Chemistry
UniProt number
  • Q9Q6P4
Molecular Mass/ Weight
  • 32 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 100 µg/ml
Purification Method
  • Purified
Purity
  • >95%
Storage & stability
Form
  • In solution
Storage Buffer
  • 25 mM Tris-HCl, pH 7.5, 150 mM NaCl
Storage Conditions
  • Store at -80°C. Avoid multiple freeze-thaw cycles. Purified recombinant protein has autocatalytic activity, which can cause protein degradation (faint 22 kDa and 11 KDa fragments can be visible on SDS PAGE). Therefore, it is important to store the product below -20° C and to keep it on ice while working with the product
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of Pyr-RTKR-AMC substrate from SensoLyte™ 440 West Nile Virus Protease Assay Kits per minute at pH 8.0 at 30° C.
Specificity
  • Recombinant WNV NS3 protease (residues 1 to 184)
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • West Nile Virus

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