Protein

West Nile Virus NS3 Protease, recombinant

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  • Cat.Number : AS-72081-5
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Size

Quantity

West Nile virus (WNV) is a member of the flavivirus genus, which contains many significant human pathogens including Dengue virus (Den), Japanese Encephalitits virus (JE), and Yellow Fever virus (YF). WNV is a small, enveloped virus with a single stranded, positive sense 11kb RNA genome, which encodes a polyprotein precursor. This polyprotein must be cleaved co- and post-translationally to produce ten functional proteins: three structural (C, prM and E) and 7 nonstructural (NS1, NS2A, NS2B, NS3, NS4A,NS4B and NS5). WNV NS3 protease is absolutely essential (along with viral-encoded cofactor NS2B) for post-translational cleavage and viral replication. As a result, this protease is a potential therapeutic target

His-tagged recombinant WNV NS3 protease (residues 1 to 184) was expressed as a fusion protein with the cofactor NS2b (residues 49 to 96) and a linker sequence (GGGGSGGGG) in E. coli. The apparent Mr on SDS-PAGE is 32-kDa. Its activity can be measured by FRET peptides

Specifications

Chemistry
UniProt number
  • Q9Q6P4
Molecular Mass/ Weight
  • 32 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 100 µg/ml
Purification Method
  • Purified
Purity
  • ≥95% by SDS-PAGE
Storage & stability
Form
  • In solution
Storage Buffer
  • 25 mM Tris-HCl, pH 7.5, 150 mM NaCl
Storage Conditions
  • Store at -80°C. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of Pyr-RTKR-AMC substrate from SensoLyte™ 440 West Nile Virus Protease Assay Kits per minute at pH 8.0 at 30° C.
Specificity
  • Recombinant WNV NS3 protease (residues 1 to 184)
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • West Nile Virus
Codes
Code Nacres
  • NA.27

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