MMP-1 (interstitial collagenase, fibroblast collagenase) is an extracellular protease. This enzyme plays a significant role in some diseases and has been identified as a drug screening target. The SensoLyte® 490 MMP-1 Assay Kit uses an optimized fluorogenic peptide substrate for continuous measurement of the enzyme activity, providing speed, convenience and accuracy in analyzing MMP-1 and screening MMP-1 inhibitors. Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-1 activity, please choose the SensoLyte® Plus MMP-1 Assay Kit, Cat# AS-72012.
Component A: MMP-1 substrate EDANS/DabcylPlus™ FRET peptide Ex/Em=340 nm/490 nm: upon cleavage 270 µL
Component B: EDANS, fluorescence reference standard (1 mM, 10 µL), Ex/Em=340 nm/490 nm: 1 mM, 10 µL
Component C: APMA, 4-aminophenylmercuric acetate: 1 M, 100 µL
Component D: Assay buffer: 60 mL
Component E: Stop solution: 30 mL
Storage & stability
Store all components at -20°C. Protect Components A and B from light. Components C and D can be stored at 4 °C for convenience.